Review



il 13  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    R&D Systems il 13
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-6+Quantikine+ELISA+Kit/pmc13122667-77-39-40
    Average 97 stars, based on 1592 article reviews
    il 13 - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma"

    Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

    Journal: Redox Biology

    doi: 10.1016/j.redox.2026.104180

    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Figure Legend Snippet: Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Activity Assay

    Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.
    Figure Legend Snippet: Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

    Techniques Used: Over Expression, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay

    Related Articles

    other:

    Article Title: Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in mice.
    Article Snippet: Lrp1 f/f mice (No. T064127) and Csf1r-Cre mice (No. T005640) were constructed by GemPharmatech (Nanjing, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: IL-10 controls Th2-type cytokine production and eosinophil infiltration in a mouse model of allergic airway inflammation.
    Article Snippet: Interleukin-10 was originally described as a factor that inhibits cytokine production by murine Th1 clones.. Recent studies have since shown that IL-10 can also downregulate Th2 clones and their production of IL-4 and IL-5.. Because of its immuno-suppressive properties, IL-10 has been suggested as a potential therapy for allergic inflammation and asthma.

    Article Title: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis
    Article Snippet: Relevant bands were quantified with laser-scanning densitometry (Image Quant LAS 4000 mini, Image Quant TL Analysis Toolbox, GE Healthcare UK Ltd). .. The amounts of IL-4 and IL-13 in the proteins from colon tissue were measured using mouse IL-4 Quantikine ELISA kit and mouse IL-13 Quantikine ELISA kit (R&D Systems Inc.) respectively, following the manufacturers’ instructions. ..

    Article Title: Stress granule assembly impairs macrophage efferocytosis to aggravate allergic rhinitis in mice
    Article Snippet: h Working model for SG assembly-inhibited macrophage efferocytosis through Lrp1 mRNA translational arrest to aggravate AR progression, created by Figdraw (export ID: IRIRWb0667). .. Mouse IL-13 Quantikine ELISA Kit (M1300CB) and mouse IL-5 Quantikine ELISA Kit (M5000) were purchased from R&D Systems (Minneapolis, MN). ..

    Article Title: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: a possible role in ulcerative colitis.
    Article Snippet: Enzyme-linked immunosorbent assays for IL-4 and IL-13 in colon tissue. .. The amounts of IL-4 and IL-13 in the proteins from colon tissue were measured using mouse IL-4 Quantikine ELISA kit and mouse IL-13 Quantikine ELISA kit (R&D Systems Inc.) respectively, following the manufacturers’ instructions. ..

    Article Title: GATA-3 regulates contact hyperresponsiveness in a murine model of allergic dermatitis.
    Article Snippet: Contact hypersensitivity (CHS) is thought to be associated mainly with the activation of T helper (Th) type 1 cells.. However, evidence also suggests that Th type 2 cells (Th2) and cytokines play roles in the development of CHS in humans.. The present study examines the Th2 response during the development of CHS in response to 2,4,6-trinitrochlorobenzene (TNCB) in GATA-3-transgenic (GATA-3 Tg) mice.

    Article Title: Th2 immune response plays a critical role in the development of nickel-induced allergic contact dermatitis.
    Article Snippet: Background: The precise roles of T helper (Th)1-type and Th2-type cytokine responses in nickel (Ni)-induced allergic contact dermatitis have not yet been clearly defined.. We investigated the involvement of Th2 cytokines in Ni-induced contact hypersensitivity reaction using GATA-3 transgenic (Tg) mice.. Methods: A Ni-titanium (Ti) alloy was implanted under the skin of GATA-3 Tg mice.

    Article Title: Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption
    Article Snippet: The fluorescence intensity was determined by the CFX96 Real-Time PCR Detection system (Bio-Rad Laboratories, Inc.) The relative expression ratio of cytokines was calculated using the 2 −ΔΔCq method ( ). .. The protein levels of IL-5 and IL-13 in the supernatants of the colon tissue lysates were measured using Mouse IL-13 Quantikine ELISA kit and Mouse IL-5 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA), according to the manufacturer's protocol. ..



    Similar Products

    97
    R&D Systems il 13
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-6+Quantikine+ELISA+Kit/pmc13122667-77-39-40
    Average 97 stars, based on 1 article reviews
    il 13 - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    95
    R&D Systems il 13 quantikine elisa kits
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Il 13 Quantikine Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+DuoSet+ELISA/pm41453449-95-8-24
    Average 95 stars, based on 1 article reviews
    il 13 quantikine elisa kits - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    93
    R&D Systems mouse il 6 kit
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Mouse Il 6 Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+Culture+Media+Quantikine+ELISA/pm40668913-509-1-8
    Average 93 stars, based on 1 article reviews
    mouse il 6 kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems mouse il 13 quantikine elisa kit
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Mouse Il 13 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+Culture+Media+Quantikine+ELISA/pm40595582-211-0-16
    Average 93 stars, based on 1 article reviews
    mouse il 13 quantikine elisa kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems il 13 elisa kits
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Il 13 Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+R+alpha+2+Quantikine+ELISA+Kit/pmc12165274-56-4-10
    Average 93 stars, based on 1 article reviews
    il 13 elisa kits - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems mouse il 13 elisa kit quantikine
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Mouse Il 13 Elisa Kit Quantikine, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+Culture+Media+Quantikine+ELISA/pmc12094982-50-10-17
    Average 93 stars, based on 1 article reviews
    mouse il 13 elisa kit quantikine - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Bio-Techne corporation mouse il-13 culture media quantikine elisa
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Mouse Il 13 Culture Media Quantikine Elisa, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+Culture+Media+Quantikine+ELISA/bio-techne+corporation___m1300cb
    Average 94 stars, based on 1 article reviews
    mouse il-13 culture media quantikine elisa - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    93
    R&D Systems mouse il 13 elisa kit
    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, <t>and</t> <t>IL-13</t> levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.
    Mouse Il 13 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+13+quantikine+elisa+kit/Mouse+IL-13+Culture+Media+Quantikine+ELISA/pm38673859-229-4-9
    Average 93 stars, based on 1 article reviews
    mouse il 13 elisa kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

    Journal: Redox Biology

    Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

    doi: 10.1016/j.redox.2026.104180

    Figure Lengend Snippet: Effects of CuONPs on allergic inflammation and oxidative stress in OVA-induced asthmatic mice. (A) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (B–F) Total and differential inflammatory cell counts in BALF. (G–L) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (M and N) Total IgE and OVA specific IgE levels in serum. (O and P) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 6 mice/group). ∗∗ p < 0.01 and ∗∗∗ p < 0.001 versus NC group. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus OVA group.

    Article Snippet: BALF was centrifuged at 300× g for 10 min at 4 °C, and the supernatant was stored for cytokine analysis using commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantify IL-1β, IL-6, tumor necrosis factor (TNF)-α, IL-4, IL-5, and IL-13 (R&D Systems, Minneapolis, MN, USA; Cat. No. MLB00C, M6000B, MTA00B, M4000B, M5000, and M1300CB, respectively).

    Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

    Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

    Journal: Redox Biology

    Article Title: Nrf2 pathway mediates copper oxide nanoparticle-induced exacerbation of allergic asthma

    doi: 10.1016/j.redox.2026.104180

    Figure Lengend Snippet: Effects of Nrf2 overexpression on allergic inflammation and oxidative stress in CuONP-exposed asthmatic mice. (A) Immunofluorescence analysis of lung tissue from mice administered PBS or AAV2/8-GFP via intratracheal instillation. (B) Airway hyperresponsiveness assessed as total respiratory system resistance in response to methacholine challenge (10, 20, and 40 mg/mL). (C–G) Total and differential inflammatory cell counts in BALF. (H–M) IL-1β, IL-6, TNF-α, IL-4, IL-5, and IL-13 levels in BALF, measured by ELISA. (N and O) Total IgE and OVA specific IgE levels in serum. (P and Q) MDA levels and SOD activity in lung tissue. Data are presented as means ± SD (n = 3 mice/group for panels A; n = 6 mice/group for panels B–Q). In panel B, ## p < 0.01 indicates significant differences between GFP-NC and GFP-OVA, and ∗∗ p < 0.01 indicates significant differences between GFP-OVA + CuONPs and Nrf2-OVA + CuONPs. For panels C–Q, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001 indicate significant differences between AAV-GFP and AAV-Nrf2 within each condition.

    Article Snippet: BALF was centrifuged at 300× g for 10 min at 4 °C, and the supernatant was stored for cytokine analysis using commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantify IL-1β, IL-6, tumor necrosis factor (TNF)-α, IL-4, IL-5, and IL-13 (R&D Systems, Minneapolis, MN, USA; Cat. No. MLB00C, M6000B, MTA00B, M4000B, M5000, and M1300CB, respectively).

    Techniques: Over Expression, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Activity Assay